Mosquito Repellent against Anopheles Spp. and Aedes Aegypti on Cotton Fabric

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Figure 13.1: Human landing counts. A plastic glove with an opening (5 × 5 cm) on the

upper side was used. The testing material was loaded to a filter paper or treated textile (7 ×

7 cm) with an opening (5 × 5 cm) and placed in order to surround the glove opening [12].

zero landings were counted, was ≈0.2µlcm⁻². All testing materials were applied on pa-

per (Whatman chromatography paper) of 24 cm² total area and tested at two doses: 50

µl (“low”, ≈0.2µlcm⁻² of testing material) and 100 µl (“high”, ≈0.4µlcm⁻² of testing

material) of 100 µgµl⁻¹ stock solution.

The paper was placed around the glove opening (Figure 13.1). Five minutes after treat-

ment with the testing material (to ensure the solvent evaporation), the treated area (glove

with filter paper) was inserted for 5 min through the sleeve into the cage. Control treat-

ments without the components and with DEET were also included for the repellency tests

as standards (control and positive control, respectively). Each treatment was repeated eight

times and four human volunteers were used [11].

The physical structure and elemental composition of cotton fibers were analyzed by Hi-

tachi SU3500 scanning electron microscopy equipped with energy dispersive spectrometer

detector (SEM-EDS). The imaging was made with 3 kV accelerating voltage. The samples

were coated with a 10 nm thick layer of gold before analysis [13].

13.3

RESULTS

Present work attempt to find suitable natural repellents for effective mosquito control

measure and by this to contribute, in the near future, to human protection from the vector

transmitted diseases.For this purpose, natural vibroactivated zeolite, Immortelle Oil and